GLC determination of nylidrin in human urine samples

Area of Science:

• Analytical Chemistry
• Forensic Toxicology

Background:

• Nylidrin is a medication used to improve blood flow.
• Accurate detection of nylidrin in biological samples is crucial for therapeutic drug monitoring and forensic analysis.

Purpose of the Study:

• To develop and validate a sensitive method for quantifying nylidrin in human urine.
• To assess the method's applicability in real-world scenarios following oral nylidrin administration.

Main Methods:

• The method involves enzymatic hydrolysis using beta-glucuronidase.
• Extraction of nylidrin from urine using chloroform.
• Derivatization of nylidrin via silylation.
• Quantification using Gas-Liquid Chromatography (GLC).

Main Results:

• The described method is capable of detecting nanogram quantities of nylidrin.
• The method demonstrated suitability for analyzing urine samples from individuals who ingested nylidrin hydrochloride.

Conclusions:

• A reliable and sensitive analytical method for nylidrin detection in human urine has been established.
• This method can be effectively used for monitoring nylidrin levels in clinical and forensic contexts.