Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Basics of flow cytometry

G Radcliff1, M J Jaroszeski

  • 1Moffitt Research Center, H. Lee Moffitt Cancer Center and Research Institute, University of South Florida, Tampa, FL, USA.

Methods in Molecular Biology (Clifton, N.J.)
|July 17, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clinical trials for solid tumors using electrochemotherapy.

Methods in molecular medicine·2011
Same author

Delivery of genes in vivo using pulsed electric fields.

Methods in molecular medicine·2011
Same author

Electrochemotherapy of murine melanoma using intratumor drug administration.

Methods in molecular medicine·2011
Same author

Electrochemotherapy for the treatment of soft tissue sarcoma in a rat model.

Methods in molecular medicine·2011
Same author

Treatment of liver malignancies with electrochemotherapy in a rat model.

Methods in molecular medicine·2011
Same author

Electrically mediated reporter gene transfer into normal rat liver tissue.

Methods in molecular medicine·2011
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Understanding flow cytometric instrumentation is key for effective use. Familiarity with its fluidic, optical, electronic, and computer systems aids experimental design and data analysis, preventing wasted resources.

Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Cell Biology

Background:

  • Flow cytometry is a powerful technology for analyzing individual particles.
  • It relies on complex instrumentation comprising fluidic, optical, electronic, and computer systems.
  • Effective use requires fundamental knowledge of these integrated systems.

Purpose of the Study:

  • To elucidate the essential instrumentation principles of flow cytometry for beginners.
  • To highlight the importance of understanding instrument systems for experimental design.
  • To explain data analysis techniques, including histograms and bivariate plots.

Main Methods:

  • Description of the four core systems of a flow cytometer: fluidics, illumination, optics/electronics, and data/computer control.

Related Experiment Videos

  • Explanation of particle transport, light scattering, and fluorescence detection.
  • Introduction to graphical data analysis methods like histograms and bivariate plots.

    • Main Results:

    • Knowledge of laser emission wavelengths and fluorescent channel detection is critical for probe selection.
    • Understanding spectral overlap aids in fluorochrome selection for multicolor analysis.
    • Familiarity with histograms and bivariate plots is essential for interpreting flow cytometry data.

    Conclusions:

    • Basic knowledge of flow cytometer instrumentation significantly improves experimental design and reagent selection.
    • Proficiency in data analysis, particularly graphical representations, is paramount for accurate interpretation.
    • Effective use of flow cytometry combines instrumentation understanding with data analysis skills.