Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Photon-counting device compatible with conventional flow cytometric data acquisition electronics

A Agronskaia1, A Florians, K O van der Werf

  • 1Department of Applied Physics, University of Twente, Enschede, The Netherlands.

Cytometry
|July 17, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Outcome of the geriatric head and neck cancer patient].

HNO·2026
Same author

Exoscopic-assisted head and neck surgery: a comparative evaluation optimizing ergonomics and workflow.

Journal of robotic surgery·2025
Same author

[Typical emergencies in otorhinolaryngology-a monocentric analysis of the seasonal course].

HNO·2022
Same author

[Oncologic follow-up in head and neck cancer patients].

HNO·2022
Same author

Performance of microvascular anastomosis with a new robotic visualization system: proof of concept.

Journal of robotic surgery·2021
Same author

[Robotics in otorhinolaryngology, head and neck surgery].

HNO·2021
Same journal

Measurement of the distribution of red blood cell deformability using an automated rheoscope.

Cytometry·2002
Same journal

Flow cytometric-based isolation of nucleated erythroid cells during maturation: an approach to cell surface antigen studies.

Cytometry·2002
Same journal

Flow cytometric immunophenotyping test for staging/monitoring neuroblastoma patients.

Cytometry·2002
Same journal

Determination of bronchoalveolar lavage leukocyte populations by flow cytometry in patients investigated for respiratory disease.

Cytometry·2002
Same journal

Multicenter clinical experience with flow cytometric method for fetomaternal hemorrhage detection.

Cytometry·2002
Same journal

Separation of live cells in different phases of the cell cycle for gene expression analysis.

Cytometry·2002
See all related articles

This study introduces a new electronic scheme for flow cytometry, enhancing the detection of extremely weak signals using photon-counting devices. This method accurately measures photon bursts from real events, distinguishing them from background noise.

Area of Science:

  • Biophotonics
  • Analytical Chemistry
  • Molecular Biology

Background:

  • Flow cytometry typically uses conventional detectors for signal measurement.
  • Measuring extremely weak signals, such as single molecules, presents a challenge in flow cytometry.
  • Background noise from fluorescence and Raman scattering can obscure weak signals.

Purpose of the Study:

  • To present an electronic scheme for improved weak signal detection in flow cytometry.
  • To enable the use of photon-counting devices (photomultiplier or avalanche photodetector) for enhanced sensitivity.
  • To accurately measure and discriminate weak signals from background noise.

Main Methods:

  • Developed an electronic scheme to convert photon-counting pulses into an analog signal.

Related Experiment Videos

  • The analog signal is continuously proportional to the number of detected photons within an integration time.
  • Integration time is set to match the object's illumination duration in the flow chamber.

    • Main Results:

    • The scheme allows photon-counting devices to measure extremely weak signals in flow cytometry.
    • It can be used as a sole detector or integrated with conventional detectors and data acquisition hardware.
    • The method effectively measures photon bursts from real events and discriminates them from background pulses.
    • Demonstrated the scheme's utility for measuring single DNA molecules.

    Conclusions:

    • The presented electronic scheme significantly enhances the capability of flow cytometry for detecting ultra-weak signals.
    • This approach improves the accurate measurement of photon bursts, crucial for applications like single-molecule analysis.
    • The scheme offers a versatile solution for improving sensitivity in flow cytometry without requiring entirely new hardware.