Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Scanning probe microscopy

R J Colton1, D R Baselt, Y F Dufrêne

  • 1Chemistry Division, Code 6177 Naval Research Laboratory, Washington, DC 20375-5342, USA. rcolton@stm2.nrl.navy.mil

Current Opinion in Chemical Biology
|July 17, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Insight into the outer membrane asymmetry of <i>P. aeruginosa</i> and the role of MlaA in modulating the lipidic composition, mechanical, biophysical, and functional membrane properties of the cell envelope.

Microbiology spectrum·2024
Same author

Treatment with incretins does not increase the risk of pancreatic diseases compared to older anti-hyperglycaemic drugs, when added to metformin: real world evidence in people with Type 2 diabetes.

Diabetic medicine : a journal of the British Diabetic Association·2018
Same author

Biofilm formation - what we can learn from recent developments.

Journal of internal medicine·2018
Same author

Micromagnet arrays enable precise manipulation of individual biological analyte-superparamagnetic bead complexes for separation and sensing.

Lab on a chip·2016
Same author

Introduction.

Postgraduate medicine·2016
Same author

The medical bookshelf.

Postgraduate medicine·2016
Same journal

Function through shape: An overview of DNA G-quadruplexes in transcriptional regulation.

Current opinion in chemical biology·2026
Same journal

Advances in tools and technologies for multiplexed bioluminescence imaging.

Current opinion in chemical biology·2026
Same journal

High-resolution molecular mapping by expansion-coupled label-free and multimodal imaging.

Current opinion in chemical biology·2026
Same journal

Recent advances in glycoconjugate-based therapeutics.

Current opinion in chemical biology·2026
Same journal

Towards better red emitters for bioimaging: Innovations in rhodamine and cyanine chemistry.

Current opinion in chemical biology·2026
Same journal

Chemigenetic fluorescent biosensors in biological imaging - New trends and advances.

Current opinion in chemical biology·2026
See all related articles

Scanning probe microscopy, particularly atomic force microscopy (AFM), is advancing in biological sciences. AFM is evolving from imaging to probing biomolecule properties and enabling new biosensor applications.

Area of Science:

  • Biophysics
  • Nanotechnology
  • Molecular Biology

Background:

  • Scanning probe microscopy, especially atomic force microscopy (AFM), has gained significant traction in biological sciences.
  • The technique is transitioning from qualitative imaging to quantitative analysis of biomolecules and cells.
  • Future applications include microinstrument development for process control and sensing.

Purpose of the Study:

  • To review recent advances in AFM instrumentation and methods for biological applications.
  • To highlight the evolving capabilities of AFM in studying biomolecular dimensions, properties, and interactions.
  • To showcase emerging applications in biosensing and structural biology.

Main Methods:

  • Atomic Force Microscopy (AFM) with a focus on tapping mode operation, including its adaptation for liquid environments.

Related Experiment Videos

  • Cryo-AFM for high-resolution imaging of biological molecules at low temperatures.
  • Force spectroscopy techniques to measure molecular recognition forces.

    • Main Results:

    • Tapping mode AFM is increasingly preferred for imaging biological molecules, with successful implementation in liquid.
    • Cryo-AFM offers improved resolution for biological samples.
    • AFM enables precise measurements of viscoelastic properties and molecular recognition forces, leading to novel biosensor concepts.
    • Structural studies include DNA mapping, imaging transcription, and analyzing drug-DNA interactions.

    Conclusions:

    • AFM is a versatile tool rapidly advancing in biological research, offering quantitative insights into molecular structure and function.
    • Ongoing developments in AFM instrumentation and methods are expanding its utility in biophysics, nanotechnology, and molecular biology.
    • AFM is poised for further evolution into sophisticated microinstruments for advanced sensing and process control applications in life sciences.