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Sphingosine-1-phosphate as an intercellular signaling molecule
1Fred Hutchinson Cancer Research Center, University of Washington, Seattle 98104, USA. yigarash@pharm.hokudai.ac.jp
Annals of the New York Academy of Sciences
|July 21, 1998
Summary
Sphingosine-1-phosphate (Sph-1-P) activates platelets externally, not internally. This lipid also inhibits cell motility by binding to specific surface receptors on F10 cells.
Area of Science:
- Biochemistry
- Cell Biology
- Lipid Signaling
Background:
- Sphingosine-1-phosphate (Sph-1-P) is recognized as an intracellular second messenger.
- Previous research identified Sph-1-P's roles in platelet activation and cell motility regulation.
Purpose of the Study:
- To investigate the mechanism of Sph-1-P-induced platelet activation.
- To elucidate the role of extracellular Sph-1-P in F10 cell motility.
- To identify potential Sph-1-P receptors on F10 cells.
Main Methods:
- Platelet activation assays using exogenous Sph-1-P and Sph.
- Utilizing Sph-1-P-immobilized glass beads to assess platelet and cell surface interactions.
- F10 cell motility assays in the presence of exogenous Sph-1-P.
Main Results:
- Exogenous Sph-1-P activated platelets, while intracellular Sph-1-P did not.
- Platelet activation was observed upon contact with Sph-1-P-coated glass beads.
- Extracellular Sph-1-P inhibited F10 cell motility via specific binding sites.
- Putative Sph-1-P receptor proteins were identified on F10 cell surfaces.
Conclusions:
- Sph-1-P activates platelets through an extracellular mechanism.
- Extracellular Sph-1-P acts as an inhibitor of F10 cell motility.
- Specific cell surface receptors mediate Sph-1-P's effects on F10 cells.