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Identified taste bud cell proliferation in the perihatching chick
D Ganchrow1, J R Ganchrow, S M Royer
1Department of Anatomy and Anthropology, Sackler Faculty of Medicine, Tel Aviv University, Israel. anatom12@post.tau.ac.il
Chemical Senses
|July 21, 1998
Summary
Developing taste bud cells in chicks were studied using high voltage electron microscopy. Dark cells, a specific type, showed the highest proliferation rates, suggesting they primarily produce more dark cells during development.
Area of Science:
- Developmental biology
- Cell biology
- Histology
Background:
- Taste bud development is crucial for feeding behavior.
- Identifying proliferating cells in developing taste buds is essential for understanding their formation.
- Previous studies have not fully elucidated the specific cell types responsible for taste bud proliferation.
Purpose of the Study:
- To identify proliferating cell types within developing chick taste buds.
- To investigate the contribution of specific cell types to taste bud formation during the perihatching period.
Main Methods:
- High voltage electron microscopic autoradiography was employed.
- Taste buds from perihatching chick embryos and hatchlings were analyzed.
- [3H]thymidine incorporation was used to label proliferating cells.
Main Results:
- Dark cells constituted 55% of all identified taste bud cells and 62% of labeled cells.
- Dark cells exhibited a significantly higher number of labeled cells and greater label incorporation per nucleus compared to other cell types.
- A positive correlation was observed between the amount of label and nuclear area specifically in dark cells.
Conclusions:
- Dark cells are the primary proliferating cell type in developing chick taste buds during the perihatching period.
- Dividing dark cells likely give rise to progeny that are also dark cells, contributing significantly to taste bud growth.
- These findings provide insights into the cellular mechanisms driving taste bud development.