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Synthesis of gamma-phosphate-linked nucleoside affinity chromatography resins for protein purification, including
R K Suto1, M A Whalen, B R Bender
1Department of Biochemistry, Colorado State University, Fort Collins 80523, USA.
Nucleosides & Nucleotides
|July 22, 1998
Abstract:
Seven nucleotides linked through the gamma-phosphate to diamine hydrocarbons were synthesized and coupled to Sepharose for use in protein purification affinity chromatography. The synthesis involved converting the nucleotides to nucleoside-5'- trimetaphosphates using dicyclohexyl carbodiimide, followed by nucleophilic ring opening of the trimetaphosphate with an alpha, omega-diamino hydrocarbon to generate a gamma-phosphoamide linkage in each nucleotide.