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Steroid binding by mouse salivary proteins

R C Karn1

  • 1Department of Biological Sciences, Butler University, Indianapolis, Indiana 46208, USA.

Biochemical Genetics
|July 23, 1998
PubMed
Summary

Mouse salivary androgen-binding protein (ABP) specifically binds testosterone and progesterone. Another salivary protein binds cholesterol, but no protein identified binds estradiol.

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Area of Science:

  • Endocrinology
  • Biochemistry
  • Proteomics

Background:

  • Salivary glands produce proteins with diverse functions.
  • Androgen-binding protein (ABP) is known to bind androgens.
  • The steroid-binding capabilities of mouse salivary proteins are not fully characterized.

Purpose of the Study:

  • To determine the steroid-binding specificity of the mouse salivary androgen-binding protein (ABP) family.
  • To identify other potential steroid-binding proteins in mouse saliva.

Main Methods:

  • Optimized testosterone binding conditions using chromatography.
  • Developed a protocol for steroid-binding specificity analysis of electrophoretically separated salivary proteins.
  • Utilized steroid structural comparisons to infer binding mechanisms.

Main Results:

  • Mouse salivary ABP family demonstrated significant binding for testosterone and progesterone, with lesser binding for hydroxyprogesterone and dihydrotestosterone (DHT).
  • Cholesterol and estradiol were not bound by the ABP family.
  • A distinct salivary protein, not part of the ABP family, specifically bound cholesterol.
  • No salivary protein was found to specifically bind estradiol.

Conclusions:

  • Mouse salivary ABP exhibits specific binding patterns, primarily for testosterone and progesterone, influenced by the steroid's A ring structure.
  • Mouse saliva contains multiple steroid-binding proteins, including a unique cholesterol-binding protein.
  • Further research is needed to identify the estradiol-binding protein in mouse saliva.

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