Related Experiment Videos
Isolation of CD34+ cells from blood stem cell components using the Baxter Isolex system
S D Rowley1, M Loken, J Radich
1Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98104, USA.
Bone Marrow Transplantation
|July 23, 1998
Summary
Immunomagnetic separation effectively enriches CD34+ cells from peripheral blood stem cells for autologous transplantation. Enriched CD34+ cells facilitate rapid engraftment in patients with B lymphoid malignancies.
Area of Science:
- Hematology
- Immunology
- Cell Biology
Background:
- CD34 antigen identifies hematopoietic progenitor and stem cells crucial for marrow reconstitution.
- Marrow-ablative chemo-radiotherapy necessitates effective stem cell sources for recovery.
- Various technologies exist for separating CD34+ cells from bone marrow and peripheral blood stem cells (PBSC).
Purpose of the Study:
- To evaluate the efficacy of immunomagnetic separation for enriching CD34+ cells from PBSC for autologous transplantation.
- To assess the engraftment potential of these enriched CD34+ cells in patients with B lymphoid malignancies.
Main Methods:
- Immunomagnetic separation technique applied to PBSC components.
- Mobilization chemotherapy followed by G-CSF administration.
- Analysis of CD34+ cell concentration, purity, recovery, and depletion of CD34- cells.
Main Results:
- 21 out of 29 patients achieved the target CD34+ cell level for processing.
- Enriched products showed high purity (90.8%) and recovery (58.4%) of CD34+ cells.
- Nineteen patients engrafted rapidly, achieving ANC >0.5 x 10(9)/l at a median of 11 days and platelet independence at 9 days.
Conclusions:
- Immunomagnetic separation is an effective method for enriching CD34+ cells from PBSC.
- Peripheral blood-derived CD34+ cells, when enriched, support rapid engraftment after autologous transplantation.
- This technique shows promise for autologous transplantation in B lymphoid malignancies.