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Molecular cloning of rat Pax4: identification of four isoforms in rat insulinoma cells
Y Tokuyama1, K Yagui, K Sakurai
1Second Department of Internal Medicine, Chiba University, School of Medicine, Japan.
Biochemical and Biophysical Research Communications
|July 24, 1998
Abstract:
Molecular cloning of rat Pax4 cDNA from a rat insulinoma cell line, RINm5F, library by PCR-based cloning strategy revealed four isoforms of the protein. Analysis of tissue distribution using Northern blotting and RT-PCR showed specific expression of Pax4 mRNA in pancreatic islets and RIN cells. RT-PCR confirmed that the mRNAs of four isoforms are expressed in RIN cells. These Pax4 variants may regulate the transcriptional activity of Pax4 during the development of pancreatic islets.
