Related Experiment Video

Updated: Oct 29, 2026

Multi-Gene Single Nucleotide Polymorphism Detection in Gastric Cancer Based on Ion Semiconductor Sequencing Platform
06:21

Multi-Gene Single Nucleotide Polymorphism Detection in Gastric Cancer Based on Ion Semiconductor Sequencing Platform

Published on: May 10, 2024

Expression of multidrug resistance gene (mdr-1) mRNA in gastric and colorectal cancers

Y Motoo1, S B Su, M T Nakatani

  • 1Department of Internal Medicine, Kanazawa University, Japan. motoo@kenroku.kanazawa-u.ac.jp

Anticancer Research
|July 25, 1998
PubMed

Insights

Multidrug resistance (mdr-1) gene mRNA expression was detected in gastric and colorectal cancers. Higher expression in cancerous tissues suggests a link between mdr-1 mRNA and cancer cell differentiation.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • The multidrug resistance (mdr-1) gene plays a crucial role in cancer chemotherapy resistance.
  • Understanding mdr-1 gene expression in gastrointestinal cancers is vital for treatment strategies.

Purpose of the Study:

  • To investigate the expression of mdr-1 gene mRNA in gastric and colorectal cancers.
  • To determine the correlation between mdr-1 mRNA expression and the degree of cellular differentiation in these cancers.

Main Methods:

  • Analysis of fresh surgical specimens from 52 gastric and 25 colorectal cancers.
  • Non-isotopic in situ hybridization (ISH) using a biotin-labeled oligonucleotide probe to detect mdr-1 mRNA.

Main Results:

  • mdr-1 mRNA was significantly higher in cancerous portions (15.4%) compared to non-cancerous portions (1.9%) of gastric cancers (p < 0.02).
  • In colorectal cancers, mdr-1 mRNA was detected in 36% of cancerous and 28% of non-cancerous portions.
  • Gastric cancers with well-differentiated histology showed a significantly higher mdr-1 mRNA positive rate than poorly differentiated types (p < 0.01).

Conclusions:

  • mdr-1 mRNA expression is elevated in both gastric and colorectal cancerous tissues.
  • The findings suggest a relationship between mdr-1 mRNA expression and the degree of cellular differentiation in gastric and colorectal cancers.

Related Concept Videos

Treatment Resistant Cancers02:56

Treatment Resistant Cancers

Cancer is the second leading cause of death in the United States. A cancer cell is genetically unstable and hence can mutate faster. They can also modify their microenvironment and escape immune surveillance. The difficulties in treating cancer are further compounded by the emergence of rapid resistance to anticancer drugs. The most common ways to attain resistance in cancer cells include alteration in drug transport and metabolism, modification of drug target, elevated DNA damage response, or...
Pharmacogenetics of Drug Targets: β₂-Adrenergic Receptors, Apo E, Thymidylate Synthase01:11

Pharmacogenetics of Drug Targets: β₂-Adrenergic Receptors, Apo E, Thymidylate Synthase

Genetic polymorphisms in drug targets have emerged as critical determinants of interindividual variability in drug response and toxicity. Pharmacogenomic investigations increasingly focus on identifying these variations to personalize and optimize therapeutic interventions. A drug target may be a receptor, enzyme, or signaling protein involved in pharmacologic responses or disease-related pathways. While early pharmacogenetic studies focused primarily on drug metabolism, current research...