Liquid chromatography with fluorimetric detection of triorganotin compounds in marine biological materials

Several extraction methods have been evaluated for marine biological materials with regard to tributyltin and triphenyltin determination by liquid chromatography with post-column fluorimetric detection using fisetin as a fluorogenic reagent. Extraction with ethyl acetate in a hydrochloric acid medium has been shown to be the most appropriate extraction method and it has been successfully applied to fish (sea bass) reference material from the National Institute for Environmental Studies (NIES-11) and to a candidate reference material.