Yeast permeabilization as a tool for measurement of in situ enzyme activity: localization of alkaline phosphatase
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Updated: Oct 29, 2026
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Measuring In Vitro ATPase Activity for Enzymatic Characterization
Published on: August 23, 2016
1Bulgarian Academy of Sciences, Institute of Microbiology, Sofia, Bulgaria.
Abstract:
The biochemical and ultracytochemical localization of alkaline phosphatases in permeabilized cells of Saccharomyces cerevisiae 257 has been studied. The treatment with non-ionic surfactant Triton X-100 allows the penetration of the substrate into intact yeast cells and thus provides detailed detection of the enzyme activity in ultracytochemical studies.
