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Updated: Aug 8, 2026

Protein Purification-free Method of Binding Affinity Determination by Microscale Thermophoresis
Published on: August 15, 2013
Selective roles for TATA-binding-protein-associated factors in vivo
1Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA.
Abstract:
Transcription factor TFIID, a central component of the eukaryotic RNA polymerase II transcription machinery, is a multiprotein complex containing the TATA-binding protein (TBP) and TBP-associated factors (TAFs). In vitro, TAFs are required for the response to activator proteins, but they are dispensible for basal transcription. However, recent work in yeast cells indicates that TAFs are not generally required for transcriptional activation, but rather have selective effects on gene expression. Molecular mechanisms for these observations are considered.
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