Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

High cell-density fermentation

J Stratton1, V Chiruvolu, M Meagher

  • 1Department of Food Science and Technology, University of Nebraska, Lincoln, USA.

Methods in Molecular Biology (Clifton, N.J.)
|July 29, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Implementation of OIE international standards: challenges and opportunities for monitoring.

Revue scientifique et technique (International Office of Epizootics)·2020
Same author

One Health operations: a critical component in the International Health Regulations Monitoring and Evaluation Framework.

Revue scientifique et technique (International Office of Epizootics)·2019
Same author

Performance of Veterinary Services Pathway evolution and One Health aspects.

Revue scientifique et technique (International Office of Epizootics)·2019
Same author

A team approach to the difficult airway.

British journal of anaesthesia·2018
Same author

Are Village Animal Health Workers Able to Assist in Strengthening Transboundary Animal Disease Control in Cambodia?

Transboundary and emerging diseases·2015
Same author

Income disparities in life expectancy in the City of Toronto and Region of Peel, Ontario.

Chronic diseases and injuries in Canada·2012
Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for Functional Validation of Terpenoid Metabolic Clusters in Nicotiana benthamiana and Aspergillus oryzae.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

This guide details essential equipment and strategies for high-cell density fermentation of Pichia pastoris (P. pastoris) yeast. It covers aeration, pH control, and nutrient feeding for successful foreign protein expression.

Area of Science:

  • Biotechnology
  • Microbial Fermentation
  • Molecular Biology

Background:

  • Pichia pastoris is a widely used yeast for recombinant protein production.
  • Optimizing fermentation conditions is crucial for maximizing foreign protein yields.

Purpose of the Study:

  • To provide a comprehensive overview of equipment and strategies for Pichia pastoris fermentation.
  • To enable readers with limited experience to perform high-cell density fermentations.

Main Methods:

  • Ensuring sufficient aeration through proper mixing and gas blending for dissolved oxygen control.
  • Implementing automatic pH control for optimal yeast growth and protein expression.
  • Utilizing peristaltic pumps and specific tubing (e.g., Teflon) for controlled nutrient feeding in fed-batch cultures.

Related Experiment Videos

Main Results:

  • High-cell density fermentation of P. pastoris can be achieved with appropriate equipment and controlled parameters.
  • Effective aeration, pH management, and nutrient feeding are key to successful foreign protein expression.
  • The described methods are adaptable to various fermentor systems beyond the specific BioFlo III model.

Conclusions:

  • Successful high-cell density fermentation of P. pastoris relies on meticulous control of aeration, pH, and feeding strategies.
  • This chapter equips readers with the knowledge to optimize P. pastoris fermentation for enhanced foreign protein production.
  • The principles discussed are broadly applicable, facilitating high expression levels in diverse fermentation setups.