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Viral inactivation of human bone tissue using supercritical fluid extraction
ASAIO Journal (American Society for Artificial Internal Organs : 1992)
|July 31, 1998
Summary
Supercritical carbon dioxide fluid extraction (SFE) effectively inactivates viruses in bone tissue. This novel process offers a safe and efficient method for processing bone allografts, enhancing transplant safety.
Area of Science:
- Biotechnology
- Virology
- Materials Science
Background:
- Bone allografts are crucial for transplantation but carry a risk of viral transmission.
- Existing methods for viral inactivation may have limitations or require harsh chemicals.
Purpose of the Study:
- To evaluate the efficacy of supercritical carbon dioxide fluid extraction (SFE) in inactivating viruses in bone tissue.
- To compare the viral inactivation capacity of SFE with traditional methods.
Main Methods:
- Four viruses (HIV-1, Sindbis, poliovirus, PRV) were exposed to supercritical CO2, hydrogen peroxide, sodium hydroxide, and ethanol.
- Viral reduction factors (log10) were quantified for each treatment step.
Main Results:
- Supercritical CO2 fluid extraction (SFE) alone achieved mean log10 reduction factors greater than 4.0 for all tested viruses.
- Combined treatments resulted in substantial viral inactivation, with factors exceeding 14.2 for HIV-1 and 24.4 for poliovirus.
- SFE demonstrated comparable viral inactivation to traditional cleaning methods.
Conclusions:
- Supercritical CO2 fluid extraction is a highly effective method for viral inactivation on human femoral heads.
- CO2 SFE can be integrated into bone allograft processing to enhance safety.
- This process offers a promising alternative or supplement to current viral inactivation procedures.