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Immunoassays based on surface-enhanced infrared absorption spectroscopy

C W Brown1, Y Li, J A Seelenbinder

  • 1Department of Chemistry, University of Rhode Island, Kingston 02881, USA.

Analytical Chemistry
|July 31, 1998
PubMed
Summary
This summary is machine-generated.

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A novel biosensor utilizing surface-enhanced infrared absorption (SEIRA) spectroscopy detects pathogens by identifying unique spectral fingerprints. This SEIRA sensor shows promise for pathogen detection and identification.

Area of Science:

  • Biosensing
  • Spectroscopy
  • Nanotechnology

Background:

  • Pathogen detection is crucial for public health.
  • Existing methods can be time-consuming and complex.
  • Development of rapid and sensitive biosensors is needed.

Purpose of the Study:

  • To develop a novel biosensor for pathogen detection.
  • To utilize surface-enhanced infrared absorption (SEIRA) spectroscopy for generating spectral fingerprints.
  • To demonstrate the sensor's capability in identifying specific pathogens.

Main Methods:

  • Immobilizing antibodies onto a gold-coated silicon wafer.
  • Utilizing SEIRA spectroscopy in external reflection mode.
  • Analyzing spectral changes upon antigen binding for glucose oxidase (GOX) and Salmonella (SAL).

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Main Results:

  • SEIRA spectra of immobilized antibodies showed characteristic bands (1085 and 990 cm-1) with p-polarized light.
  • Specific new bands were observed upon binding of GOX (1397, 1275, 930 cm-1) and SAL (1045 cm-1) antigens.
  • Changes in water absorption bands (approx. 3500 and 850 cm-1) were observed with antigen presence.

Conclusions:

  • The developed SEIRA biosensor can generate unique spectral fingerprints for specific pathogens.
  • The sensor demonstrates potential for sensitive and specific detection of analytes like GOX and SAL.
  • SEIRA spectroscopy offers a promising platform for label-free biosensing applications.