Related Experiment Videos
Adeno-associated virus-mediated gene transfer into human retinal pigment epithelium cells
Y K Lai1, P Rakoczy, I Constable
1Centre for Ophthalmology and Visual Science, University of Western Australia, Perth, Australia. ylai@cyllene.uwa.edu.au
Purpose:
Adeno-associated virus (AAV) is emerging as a promising vector for gene therapy
Method:
To determine the ability of recombinant AAV (rAAV) to express and integrate exogenous DNA into human retinal pigment epithelium (RPE) cells, a rAAV-GFP vector containing the green fluorescent protein (gfp) and neomycin resistance (neo(r)) genes was constructed and used to transduce RPE 407A cell line.
Results:
Fluorescent RPE cell clones were obtained and were confirmed to still be expressing GFP after 24 passages (3.5 months).
Conclusion:
Adeno-associated virus-based vectors are able to efficiently transduce and stably persist in RPE cells.