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Major histocompatibility complex class II expression by intrinsic renal cells is required for crescentic
1Centre for Inflammatory Diseases, Monash University Department of Medicine, Monash Medical Centre, Clayton, 3168, Victoria, Australia.
The Journal of Experimental Medicine
|August 4, 1998
Summary
Major histocompatibility complex class II (MHC II) is essential for initiating immune renal injury in CD4(+) T cell-dependent glomerulonephritis. Intrinsic renal cells expressing MHC II are crucial for this cell-mediated kidney damage.
Area of Science:
- Immunology
- Nephrology
- Cell Biology
Background:
- Immune renal injury, specifically crescentic glomerulonephritis (GN), is often mediated by CD4(+) T cells.
- The precise role of major histocompatibility complex class II (MHC II) in initiating this process has not been fully elucidated.
Purpose of the Study:
- To investigate the requirement of MHC II for the initiation of CD4(+) T cell-dependent crescentic GN.
- To determine if MHC II expression on intrinsic renal cells is necessary for immune-mediated renal injury.
Main Methods:
- Utilized a murine model of CD4(+) T cell-dependent crescentic GN induced by sheep globulin.
- Employed MHC II-deficient mice (MHC II-/-) and chimeric mice with selective MHC II expression.
- Assessed glomerular CD4(+) T cell infiltration, renal injury, and immune responses.
Main Results:
- MHC II-sufficient mice developed crescentic GN, while MHC II-deficient mice did not.
- Chimeric mice lacking MHC II on intrinsic renal cells did not develop GN despite systemic immune responses.
- These findings indicate MHC II is critical for initiating immune renal injury.
Conclusions:
- Major histocompatibility complex class II (MHC II) is indispensable for the initiation of CD4(+) T cell-dependent crescentic glomerulonephritis.
- Interaction between CD4(+) T cells and intrinsic renal cells expressing MHC II is a prerequisite for cell-mediated immune renal injury.