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Blastocyst development from supernumerary embryos after intracytoplasmic sperm injection: a paternal influence?
Y Shoukir1, D Chardonnens, A Campana
1Clinic of Infertility and Gynaecological Endocrinology-WHO Collaborating Centre in Human Reproduction, Department of Obstetrics and Gynaecology, University Hospital of Geneva, Switzerland.
Human Reproduction (Oxford, England)
|August 4, 1998
Summary
Poor sperm motility, not concentration or morphology, impacts embryo development after intracytoplasmic sperm injection (ICSI). In-vitro fertilization (IVF) shows higher blastocyst development rates than ICSI, suggesting a paternal effect on early embryogenesis.
Area of Science:
- Reproductive Biology
- Human Embryogenesis
- Assisted Reproductive Technologies
Background:
- Intracytoplasmic sperm injection (ICSI) is a key assisted reproductive technology.
- The influence of paternal factors on early human embryogenesis requires further investigation.
- Understanding sperm parameters' role in embryo development is crucial for improving ICSI outcomes.
Purpose of the Study:
- To investigate the impact of poor sperm parameters on the development of ICSI-fertilized embryos to the blastocyst stage.
- To compare blastocyst development rates between ICSI and in-vitro fertilization (IVF).
- To determine if abnormal spermatozoa negatively affect preimplantation embryo development.
Main Methods:
- Development of supernumerary ICSI-fertilized embryos to the blastocyst stage was examined.
- Embryo co-culture was performed on monkey kidney epithelial cells.
- Blastocyst development rates were compared between ICSI and IVF groups, analyzing sperm parameters like concentration, morphology, and motility.
Main Results:
- Of 168 supernumerary ICSI embryos, 45 (26.8%) developed to blastocysts.
- Sperm concentration and morphology did not significantly affect blastocyst development.
- Higher forward progressive motility was associated with successful blastocyst formation (P = 0.015).
- Overall blastocyst development rate was lower for ICSI (26.8%) compared to IVF (47.3%).
- IVF patients with ≥3 supernumerary embryos had significantly higher blastocyst development rates (45.6%) than ICSI patients (30.0%).
- No significant difference in mean cell number or quality of supernumerary embryos between IVF and ICSI.
Conclusions:
- Abnormal spermatozoa can exert a negative paternal effect on preimplantation embryo development.
- Sperm motility is a critical factor influencing ICSI success and blastocyst development.
- IVF demonstrates superior blastocyst development rates compared to ICSI, highlighting potential paternal contributions to embryo quality.