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Updated: Aug 17, 2026

ampliPHOX Colorimetric Detection on a DNA Microarray for Influenza
Published on: June 9, 2011
Structural analysis of the amplified IFN-beta and DHFR genes in a Chinese hamster ovary cell line using multicolour
C Dixkens1, G Posseckert, T Keller
1Abteilung Medizinische Genetik, Universität Ulm.
Abstract:
Multicolour FISH was used to get insight into the structural arrangement of a homogeneously staining region which bears the co-transfected and subsequently co-amplified IFN-beta and DHFR genes in a CHO cell line. On metaphase chromosomes an arrangement of multiple bands with regular spacing is revealed. On extended chromatin fibres a cluster of directly repeated and interspersed IFN-beta and DHFR genes is visible. Up to three clusters were found arranged in tandem. The different chromosomal mechanisms leading to gene amplification are discussed.

