Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Decrease in mitochondrial energy coupling by thyroid hormones: a physiological effect rather than a pathological

V Bobyleva1, T L Pazienza, R Maseroli

  • 1Department of Biomedical Sciences, University of Modena, Italy.

FEBS Letters
|August 4, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Heme Oxygenase-1/High Mobility Group Box 1 Pathway May Have a Possible Role in COVID-19 ARDS (Acute Respiratory Distress Syndrome): A Pilot Histological Study.

Advances in experimental medicine and biology·2022
Same author

Corrigendum to: The Traveller's Risk Perception (TRiP) questionnaire: pre-travel assessment and post-travel changes.

International health·2019
Same author

The Traveller's Risk Perception (TRiP) questionnaire: pre-travel assessment and post-travel changes.

International health·2019
Same author

Comparative proteomic analysis between the gingival crevicular fluid and the corresponding periodontal pocket: a preliminary study.

Journal of biological regulators and homeostatic agents·2019
Same author

Seminal cell-free DNA molecular profile as a novel diagnostic and prognostic prostate cancer biomarkers.

Medical hypotheses·2018
Same author

Ageing and inflammation in patients with HIV infection.

Clinical and experimental immunology·2016
Same journal

Identification of a Shiga toxin A-derived peptide internalized into Gb3 receptor-bearing cells via interaction with the Shiga toxin B subunit.

FEBS letters·2026
Same journal

The dual role of lectins in cancer-immunotherapy tools and therapeutic targets.

FEBS letters·2026
Same journal

Decoding the dynamic extracellular matrix in cancer-3D models and bioscaffolds rewire the rules of tumor progression.

FEBS letters·2026
Same journal

Extending the classical sequence-structure-function paradigm through protein dynamics and context-dependent behavior.

FEBS letters·2026
Same journal

α-Synuclein aggregation landscape from phase separation to neurotoxic intermediates.

FEBS letters·2026
Same journal

Modelling stem cell differentiation related processes-A practical overview for biologists.

FEBS letters·2026
See all related articles

Thyroid status impacts liver cell mitochondria. Hypothyroidism increases mitochondrial membrane potential, while hyperthyroidism lowers it, affecting energy coupling in hepatocytes.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Endocrinology

Background:

  • Thyroid hormones are crucial regulators of cellular metabolism.
  • Mitochondrial function is central to cellular energy production.
  • The precise impact of thyroid status on mitochondrial membrane potential and energy coupling in hepatocytes requires further elucidation.

Purpose of the Study:

  • To investigate the effect of in vivo thyroid status on mitochondrial membrane potential (Δψm) in isolated rat hepatocytes.
  • To assess the relationship between thyroid status, mitochondrial membrane potential, and cellular respiration rates.
  • To explore the potential role of 6-ketocholestanol in modulating thyroid hormone-induced changes in mitochondrial function.

Main Methods:

  • Utilized a cytofluorimetric technique with the JC-1 probe to measure mitochondrial membrane potential (Δψm) in isolated rat hepatocytes.

Related Experiment Videos

  • Employed polarographic measurements to determine hepatocyte respiratory rates.
  • Administered 6-ketocholestanol and FCCP to assess their effects on mitochondrial function under varying thyroid states.

    • Main Results:

    • Mitochondrial membrane potential (Δψm) decreased in the order: hypothyroid > euthyroid > hyperthyroid.
    • Hepatocyte respiratory rates showed an inverse trend: hypothyroid < euthyroid < hyperthyroid.
    • Thyroid hormones decreased mitochondrial energy coupling, an effect not fully counterbalanced by 6-ketocholestanol, though it could recouple FCCP-uncoupled respiration.

    Conclusions:

    • Thyroid status significantly alters mitochondrial membrane potential and energy coupling in rat hepatocytes.
    • Hyperthyroidism is associated with decreased mitochondrial energy coupling, while hypothyroidism shows the opposite trend.
    • The findings suggest a complex interplay between thyroid hormones, mitochondrial function, and cellular energy metabolism, with implications for physiological regulation.