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Related Experiment Videos

Short-chain fatty acids regulate IGF-binding protein secretion by intestinal epithelial cells

A Nishimura1, M Fujimoto, S Oguchi

  • 1Developmental Gastroenterology Laboratory, Combined Program in Pediatric Gastroenterology and Nutrition, Massachusetts General Hospital, Harvard Clinical Nutrition Research Center, Charlestown, Massachusetts 02129, USA.

The American Journal of Physiology
|August 5, 1998
PubMed
Summary

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Short-chain fatty acids, like butyrate, alter gastrointestinal epithelial cells' secretion of insulin-like growth factor-binding proteins (IGFBPs). This impacts cell signaling and is linked to histone acetylation.

Area of Science:

  • Gastroenterology
  • Molecular Biology
  • Cell Biology

Background:

  • Gastrointestinal epithelial cells secrete insulin-like growth factor (IGF)-binding proteins (IGFBPs).
  • IGFBPs regulate IGF actions on cell proliferation and differentiation.
  • Short-chain fatty acids (SCFAs) are bacterial metabolites from dietary carbohydrates.

Purpose of the Study:

  • To investigate if SCFAs alter IGFBP secretion patterns in gastrointestinal epithelial cells.
  • To understand the mechanism by which luminal molecules regulate epithelial cell signaling.

Main Methods:

  • Utilized the Caco-2 intestinal epithelial cell line.
  • Administered butyrate (an SCFA) and trichostatin A.
  • Measured IGFBP secretion, mRNA accumulation, and histone acetylation.

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Main Results:

  • Butyrate increased IGFBP-2 secretion and decreased IGFBP-3 secretion in a dose-dependent and reversible manner.
  • Changes in IGFBP secretion were due to altered synthesis, not cell sorting.
  • SCFA and trichostatin A stimulation of IGFBP-2 correlated with histone acetylation.

Conclusions:

  • Intestinal epithelial cells modify IGFBP secretion in response to SCFAs.
  • This suggests a mechanism for luminal molecule regulation of gastrointestinal cell signaling.
  • Histone acetylation may play a role in SCFA-mediated IGFBP regulation.