Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Prothymosin alpha is not found in yeast

M W Trumbore1, R E Manrow, S L Berger

  • 1Division of Basic Sciences, National Cancer Institute, Bethesda, Maryland, 20892, USA.

Protein Expression and Purification
|August 7, 1998
PubMed
Summary

Prothymosin alpha, previously thought to be highly conserved, was not found in yeast or other non-mammalian organisms. This study challenges the evolutionary conservation of prothymosin alpha across diverse species.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Alcohol metabolism contributes to brain histone acetylation.

Nature·2019
Same author

Exploring the Dynamic Relationship Between Cellular Metabolism and Chromatin Structure Using SILAC-Mass Spec and ChIP-Sequencing.

Methods in enzymology·2016
Same author

Depletion of the novel p53-target gene carnitine palmitoyltransferase 1C delays tumor growth in the neurofibromatosis type I tumor model.

Cell death and differentiation·2013
Same author

Cell signaling and transcriptional regulation via histone phosphorylation.

Cold Spring Harbor symposia on quantitative biology·2011
Same author

Histone post-translational modifications regulate transcription and silent chromatin in Saccharomyces cerevisiae.

Ernst Schering Research Foundation workshop·2006
Same author

Histone H2B ubiquitylation and deubiquitylation in genomic regulation.

Cold Spring Harbor symposia on quantitative biology·2005

Area of Science:

  • Molecular Biology
  • Evolutionary Biology
  • Biochemistry

Background:

  • Prothymosin alpha is reported to be highly conserved across species, from yeast to humans.
  • Previous research suggested the presence of prothymosin alpha in yeast.

Purpose of the Study:

  • To investigate the evolutionary conservation of prothymosin alpha.
  • To determine the presence of prothymosin alpha in yeast and other non-mammalian organisms.

Main Methods:

  • Hybridization assays using yeast mRNA and genomic DNA with human prothymosin alpha probes.
  • Polymerase chain reaction (PCR) with yeast genomic DNA and human prothymosin alpha primers.
  • Yeast protein isolation and analysis.
  • Bioinformatic analysis of the Saccharomyces cerevisiae genome database using BLASTp.

Main Results:

  • Three biochemical approaches failed to detect prothymosin alpha in yeast.
  • Bioinformatic analysis confirmed the absence of a prothymosin alpha homologue in yeast.
  • Hybridization studies showed no cross-reactivity with DNA from bacteria to amphibians.

Conclusions:

  • The evolutionary conservation of prothymosin alpha from yeast to humans is not supported by this study.
  • Prothymosin alpha appears to be absent in yeast and other non-mammalian organisms examined.
  • The presence of a prothymosin alpha gene in non-mammalian animals is unlikely.

Related Experiment Videos