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Cloning of the mouse SSTR5 gene
S Moldovan1, F DeMayo, F C Brunicardi
1Department of Surgery, Baylor College of Medicine, Houston, Texas 77030, USA.
The Journal of Surgical Research
|August 8, 1998
Summary
Researchers successfully cloned the mouse somatostatin receptor subtype 5 (SSTR5) gene. This achievement is crucial for understanding SSTR5
Area of Science:
- Molecular Biology
- Genetics
- Pharmacology
Background:
- Five somatostatin receptor subtypes (SSTR1-5) are known in mammals.
- The mouse SSTR5 gene remained uncharacterized until this study.
- Cloning receptor genes enables detailed molecular analysis of their expression and function.
Purpose of the Study:
- To clone and characterize the mouse SSTR5 gene.
- To facilitate molecular investigations into SSTR5 function in a key mammalian model.
- To enable future genetic studies in mice.
Main Methods:
- Screening a mouse 129SvJ genomic library using a PCR-amplified probe from the SSTR2 gene.
- Utilizing DNA sequencing to determine the nucleotide sequence of positive clones.
- Analyzing sequence homology using the Basic Local Alignment Search Tool (BLAST).
Main Results:
- Successfully isolated and sequenced the mouse SSTR5 gene.
- The cloned sequence exhibited 92% homology to rat SSTR5 and 80% homology to human SSTR5.
- Identified 22 initial positive clones, refining to 3 highly similar clones.
Conclusions:
- The mouse SSTR5 gene has been successfully cloned and characterized.
- This cloned gene is vital for future gene regulation and ablation studies in mice.
- Understanding SSTR5's role in murine physiology and development will be advanced.