Related Experiment Video
Updated: Aug 10, 2026

A High-content Imaging Workflow to Study Grb2 Signaling Complexes by Expression Cloning
Published on: October 30, 2012
GFP illuminates the cytoskeleton
Abstract:
Until recently, cytoskeleton research has relied primarily on immunofluorescence microscopy techniques, requiring fixation and hence killing of the specimen before the analysis. The sole method for visualizing cytoskeletal dynamics in living cells has been the microinjection of purified and fluorescently labelled protein, but technical difficulties have precluded its widespread use. The recent introduction of green fluorescent protein (GFP) has enabled visualization of proteins and cytoskeletal dynamics with only minimal perturbations of the living cell and has opened new horizons for studying the cytoskeleton.
More Related Videos
Related Concept Videos
Studying the Cytoskeleton
Reporter Genes
Commonly used reporter...
Protein Dynamics in Living Cells
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

