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Solid-phase extraction method with high-performance liquid chromatography and electrochemical detection for the
A W Wright1, J A Lawrence, M Iu
1School of Pharmacy, The University of Queensland, St. Lucia, Brisbane, Australia.
Summary
A new solid-phase extraction method accurately quantifies oxycodone in human plasma. This validated technique offers high recovery and reproducibility for precise oxycodone analysis.
Area of Science:
- Analytical Chemistry
- Forensic Toxicology
- Pharmacokinetics
Background:
- Accurate quantification of oxycodone in biological matrices is crucial for clinical and forensic applications.
- Existing methods for oxycodone analysis may lack sensitivity, reproducibility, or require complex sample preparation.
Purpose of the Study:
- To develop and validate a sensitive and reproducible solid-phase extraction (SPE) method for oxycodone quantification in human plasma.
- To optimize SPE conditions for efficient extraction and recovery of oxycodone and an internal standard.
Main Methods:
- Solid-phase extraction (SPE) using Varian Certify cartridges (C8 and benzoic acid functional groups).
- Optimization of elution mobile phase (butyl chloride-isopropanol with ammonia).
- Quantification using validated SPE method with codeine as internal standard.
Main Results:
- Consistently high (> or =80%) and reproducible recoveries for oxycodone and codeine.
- Quantification limit of 5.3 pmol on-column for oxycodone.
- Low within-day (1.2-9.5%) and inter-day (6.2-6.8%) coefficients of variation.
Conclusions:
- The developed SPE method is sensitive, reproducible, and suitable for oxycodone quantification in human plasma.
- This method provides reliable results for pharmacokinetic and toxicological studies.
- The validated SPE technique enhances the accuracy of oxycodone measurement in clinical settings.