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Chromosomal assignment of two putative canine keratin gene clusters
R C Keller1, M Switonski, H Jörg
1Institute of Veterinary Biochemistry, University of Zurich, Switzerland.
Animal Genetics
|August 12, 1998
Summary
Researchers mapped canine keratin gene clusters, identifying the acidic keratin gene cluster on chromosome 20 and the basic keratin gene cluster on an unassigned autosome. This advances understanding of inherited skin disorders in dogs.
Area of Science:
- Genetics
- Comparative Genomics
- Dermatology
Background:
- Mutations in human keratin genes cause inherited keratodermas.
- Keratin genes are organized in clusters on human chromosomes 12 (basic) and 17 (acidic).
Purpose of the Study:
- To map the locations of canine keratin gene clusters.
- To facilitate further research into canine inherited skin diseases.
Main Methods:
- Used canine cosmid clones for gene mapping.
- Employed fluorescence in situ hybridization (FISH) for precise chromosomal localization.
Main Results:
- Successfully mapped the canine acidic keratin gene cluster to dog chromosome 20.
- Localized the canine basic keratin gene cluster to a small, unassigned canine autosome.
Conclusions:
- Established the chromosomal locations of canine keratin gene clusters.
- Provides a foundation for investigating the genetic basis of canine keratodermas.