[Insulin and glucagon secretion in the isolated perfused rat pancreas]

For the study of insulin and glucagon secretion at the isolated perfused rat pancreas are used, as a rule, synthetic perfusion media that have to meet two requirements: They have to ensure the biological function of the perfused organ for 2 hrs, and must not interfere with radioimmunological insulin and glucagon determination. An appropriate medium is the Krebs-Ringer bicarbonate buffer at pH 7.4 with addition of 4% dextran (MG 75000) and 0.5% human serum albumin; it allows one to produce, after permanent glucose stimulation, a biphasic insulin secretion. Infusion media based on gelatine or polyvinylpyrrolidone proved inadequate for these studies.