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Comparative genomic hybridization with lissamine- and fluorescein-labeled nucleotides
C Rosenberg1, R P van Gijlswijk, C B Vos
1Department of Molecular Cell Biology, Leiden University Medical Center, The Netherlands. carla@ruly46.leidenuniv.nl
Cytometry
|August 13, 1998
Abstract:
Biotin deoxyuridine 5'-triphosphate (dUTP) and digoxigenin dUTP are the labels most commonly used in comparative genomic hybridization (CGH). The relative infrequent use of direct fluorochrome-labeled nucleotides in CGH is related to the lower sensitivity they provide. Here we report the evaluation of two fluorochrome-conjugated nucleotides that have not been previously used in CGH (lissamine-5-dUTP and fluorescein-N6dATP) and show that this direct label combination performs at least as well as the indirect biotin/digoxigenin pair.