Related Experiment Videos
Synthesis, biochemical and biological studies on oligonucleotides bearing a lipophilic dimethoxytrityl group
K Misiura1, M Wójcik, A Krakowiak
1Department of Bioorganic Chemistry, Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Lódź. kmisiura@bio.cbmm.lodz.pl
Acta Biochimica Polonica
|August 13, 1998
Abstract:
Dimethoxytritylphosphono-oligonucleotide conjugates have been prepared. They are totally resistant to nucleases present in human serum and do not affect cleavage of a complementary oligoribonucleotide by RNase H. Conjugates possessing a phosphate backbone gave better antisense inhibition of expression of plasminogen activator inhibitor type-1 within endothelial cells as compared with unconjugated oligonucleotides.