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Use of 5-nitroindole-2'-deoxyribose-5'-triphosphate for labelling and detection of oligonucleotides
C L Smith1, A C Simmonds, A L Hamilton
1Amersham International plc, Amersham Laboratories, Buckinghamshire, UK.
Nucleosides & Nucleotides
|August 26, 1998
Abstract:
The 5'-triphosphate of 5-nitroindole-2'-deoxyriboside has been shown to be a good substrate for terminal deoxynucleotidyl transferase (TdT). An antibody has been prepared for the detection of 5-nitroindole and has been used for the detection of 5-nitroindole tailed DNA both in single-stranded form and after hybridisation to a template. This is therefore a new method for the detection of nucleic acid probes.