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A monoclonal antibody recognizing the activation domain of protein C in its calcium-free conformation

A Vincenot1, J L Pittet, M Aiach

  • 1INSERM U 428, Faculté de Pharmacie, Paris, France.

FEBS Letters
|August 26, 1998
PubMed

A monoclonal antibody (mAb) binding to protein C (PC) heavy chain but not to activated PC was found to inhibit PC activation by free thrombin, suggesting that epitope involved the activation site. Using a set of overlapping synthetic peptides, we confirmed that this mAb recognizes the sequence encompassing the thrombin cleavage site (165QVDPRLI(171)). Surprisingly, epitope was only accessible in the absence of calcium, half-maximal inhibition of mAb binding occurring at 100 microM Ca2+. Thus, our antibody provides direct evidence that conformation and/or accessibility of the activation site differ between the apo and Ca2+-stabilized conformers of PC.

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