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[Elimination of a paramyxo-type virus from oncornavirus-producing human cell lines (author's transl)]
Abstract:
A virus of the paramyxo-type was eliminated from cell-free material of human oncornavirus-producing cell lines (PMF). After transmission of this paramyxovirus-free inoculum to a human permanent cell strain (Tu 197/Tr 1) oncornaviruses were permanently formed and no paramyxoviruses could be detected. The paramyxovirus-free, oncornavirus-producing PMF-39 cell line could be established after inoculation of the TU 197/Tr 1 line with cell-free material containing both oncorna- and paramyxovirus diluted 1 to 1000. A second way of elimination of the paramyxovirus was the treatment of cell-free material containing both viruses with antisera against paramyxovirus. In the Tu 197/Tr 1 line inoculated with such material only oncornaviruses were formed. The second paramyxovirus-free oncornavirus-producing cell line was designated PMF 50.
Insights
Researchers successfully eliminated paramyxoviruses from human oncornavirus cell lines, creating new virus-free cell lines for oncornavirus research. This advancement aids in studying human oncornaviruses without contamination.
Area of Science:
- Virology
- Cell Biology
- Oncology
Context:
- Human oncornaviruses are implicated in various cancers.
- Contamination with other viruses, such as paramyxoviruses, can complicate research.
- Developing pure oncornavirus-producing cell lines is crucial for accurate study.
Purpose:
- To develop methods for eliminating paramyxoviruses from human oncornavirus-producing cell lines.
- To establish stable, paramyxovirus-free cell lines for oncornavirus research.
- To enable the isolation and study of human oncornaviruses.
Summary:
- Two methods were employed to eliminate paramyxoviruses: dilution of infectious material and treatment with specific antisera.
- Paramyxovirus-free human oncornavirus-producing cell lines (PMF-39 and PMF 50) were successfully established.
- These cell lines consistently produced oncornaviruses without detectable paramyxovirus contamination.
Impact:
- Provides researchers with reliable tools for studying human oncornaviruses.
- Facilitates a deeper understanding of oncornavirus replication and oncogenic potential.
- Contributes to the development of diagnostic and therapeutic strategies for oncornavirus-associated diseases.