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Related Experiment Videos

Progesterone determination in equine plasma using different immunoassays

P Nagy1, L Solti, M Kulcsár

  • 1Department of Obstetrics and Reproduction, University of Veterinary Science, Budapest, Hungary.

Acta Veterinaria Hungarica
|August 26, 1998
PubMed
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This study compared different assays for measuring mare plasma progesterone. Microplate ELISA showed high correlation with radioimmunoassay (RIA) and accurately detected ovulation timing, proving a reliable alternative for progesterone analysis in mares.

Area of Science:

  • Veterinary Medicine
  • Endocrinology
  • Reproductive Biology

Background:

  • Accurate measurement of plasma progesterone is crucial for reproductive management in mares.
  • Various assay systems exist, including radioimmunoassay (RIA) and enzyme-linked immunoassay (ELISA), each with potential advantages and limitations.

Purpose of the Study:

  • To compare the accuracy and reliability of different assay systems for measuring plasma progesterone concentrations in mares.
  • To evaluate the performance of direct RIA, microplate ELISA, and qualitative ELISA (tube test) against established methods.

Main Methods:

  • Plasma progesterone was measured using 3H radioimmunoassay (with and without extraction), microplate enzyme-linked immunoassay (ELISA), and qualitative ELISA (tube test).
  • Assays were compared using samples from postpartum mares, cycling mares, and mares undergoing ovulation induction.

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  • Correlation coefficients (R), sensitivity, specificity, and predictive values were calculated.

    • Main Results:

    • Direct RIA showed high correlation (R=0.94) with extraction RIA.
    • Microplate ELISA yielded higher progesterone values than RIA but accurately detected ovulation timing, with high correlations (R=0.71 and R=0.90) in different studies.
    • Qualitative ELISA (tube test) demonstrated high sensitivity (79.7%) and specificity (95.4%) compared to microplate ELISA.

    Conclusions:

    • Microplate ELISA is a reliable and accurate method for determining plasma progesterone in mares, comparable to RIA.
    • The qualitative ELISA (tube test) offers a practical and effective diagnostic tool for progesterone assessment in mares.
    • These validated assays support improved reproductive management strategies in equine practice.