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Related Experiment Videos

Versatile epitope tagging vector for gene expression in mammalian cells

T Hosfield1, Q Lu

  • 1Stratagene Cloning Systems, Inc., LaJolla, CA, USA.

Biotechniques
|August 26, 1998
PubMed
Summary

Researchers can now efficiently characterize gene products in vivo using the new pCMV-Tag1 epitope-tagging vector. This tool facilitates N-terminal, C-terminal, and internal tagging for mammalian cell gene expression studies.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Epitope tagging is crucial for protein characterization in molecular biology.
  • Existing methods may have limitations in flexibility and efficiency for in vivo studies.

Purpose of the Study:

  • To develop a versatile epitope-tagging vector for gene expression in mammalian cells.
  • To enable efficient in vivo characterization of gene products using FLAG and/or c-myc epitopes.

Main Methods:

  • Construction of the pCMV-Tag1 vector.
  • Application of the vector for N-terminal, C-terminal, and internal tagging.
  • Gene expression and characterization in mammalian cells.

Main Results:

  • Successful construction of the pCMV-Tag1 epitope-tagging vector.

Related Experiment Videos

  • Demonstration of its capability for flexible epitope tagging (N-terminal, C-terminal, internal).
  • Facilitation of rapid and efficient in vivo characterization of tagged gene products.

    • Conclusions:

    • The pCMV-Tag1 vector provides a powerful tool for molecular biologists.
    • It enhances the ability to study gene products within their native cellular environment.
    • This vector streamlines the process of protein characterization in mammalian systems.