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Updated: Aug 16, 2026

Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA
Published on: September 11, 2013
Binding and the nature of Cu(II) ion interaction with nucleosomes
E S Gelagutashvili1, K I Sigua, N A Sapojnikova
1Institute of Physics, Georgian Academy of Sciences, Tbilisi, Georgia.
Abstract:
The energetics of Cu (II) ion binding to mononucleosomes from C3HA mice liver and ascitic hepatoma 22A cells was determined from their binding isotherms by equilibrium dialysis and pulse high frequency inductively coupled plasma atomic emission spectroscopy. Anticooperative binding of copper ions with normal and tumor mononucleosomes were observed under various NaCl concentrations (0.002; 0.02; 0.2 M). The binding constants of Cu(II) ion with normal mononucleosomes in 0.002, 0.02, 0.2 M NaCl are 6.10 x 10(4), 5.22 x 10(4), 4.31 x 10(4) respectively. The binding constants of Cu(II) ion with tumor mononucleosomes in 0.002, 0.02, 0.2 M NaCl are 6.68 x 10(4), 6.12 x 10(4), 4.82 x 10(4) respectively.
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