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[Evaluation of the hemodynamic effects of tumor necrosis factor alpha factor in rats with portal hypertension using
M Pérez-Páramo1, J Muñoz, A Albillos
1Servicio de Medicina Nuclear, Clínica Puerta de Hierro, Madrid, 28035, España.
Unlabelled:
This study was aimed at investigating the effects of blocking circulating TNF on the hyperdynamic circulatory state developed in rats with portal vein stenosis (PVS), and the factors mediating the hemodynamic action of this cytokine in this setting.
Materials And Methods:
Murine antiTNF polyclonal antibodies (100 microgram) or placebo were intravenously injected into PVS rats (n = 24) before and 4 days after PVS. Hemodynamic studies were performed the day after the last antiTNF injection.
Results:
Short-term TNF inhibition led to reductions in cardiac index (p < 0.05) and portal venous inflow (p < 0.01), and significant increases in splanchnic and systemic vascular resistances. Portal pressure was unchanged, but portal-systemic shunting was decreased (p < 0.05).
Conclusion:
TNF plays a key role in promoting the development of the hyperdynamic state of portal hypertension. The effect of TNF is mediated through an increased release of nitric oxide.