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Related Experiment Videos

Nickel sequestering by polyphosphate bodies in Staphylococcus aureus

H Gonzalez1, T E Jensen

  • 1Department of Biological Sciences, Lehman College, City University of New York, Bronx 10468, USA.

Microbios
|August 29, 1998
PubMed
Summary

Staphylococcus aureus binds nickel within polyphosphate bodies, not cell walls or cytoplasm. Higher nickel concentrations led to increased metal accumulation in these specific cellular compartments.

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Area of Science:

  • Microbiology
  • Biochemistry
  • Environmental Science

Background:

  • Nickel is an essential trace element for some organisms but can be toxic at higher concentrations.
  • Understanding metal-bacteria interactions is crucial for environmental remediation and understanding pathogenesis.

Purpose of the Study:

  • To investigate nickel (Ni) uptake and intracellular localization in Staphylococcus aureus.
  • To determine if Staphylococcus aureus can compartmentalize nickel within its cellular structures.

Main Methods:

  • Staphylococcus aureus cells were exposed to varying nickel concentrations (10-100 ppm).
  • Cells were analyzed using transmission electron microscopy (TEM) in scanning transmission mode.
  • Energy-dispersive X-ray spectrometry (EDS) was employed to map nickel distribution within cells.

Main Results:

  • Polyphosphate bodies within Staphylococcus aureus cells demonstrated significant nickel binding.
  • Nickel accumulation in polyphosphate bodies correlated positively with external nickel concentrations.
  • No nickel was detected in the cell wall or cytoplasmic regions of the bacteria.

Conclusions:

  • Staphylococcus aureus sequesters nickel primarily in polyphosphate bodies.
  • This compartmentalization suggests a potential detoxification or storage mechanism for nickel.
  • Findings offer insights into nickel transport and potential implications for staphylococcal infections.

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