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Related Experiment Videos

Genetic variation within a parthenogenetic lineage

G Lushai1, P J De Barro, O David

  • 1University of Southampton, School of Biological Sciences, Biodiversity and Ecology Division, UK. GL@soton.ac.uk

Insect Molecular Biology
|September 2, 1998
PubMed
Summary

This study tracked genetic changes in a grain aphid clone over 32 generations. Researchers found evidence of both germ-line and somatic mutations, indicating genetic instability within this animal clonal lineage.

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Area of Science:

  • Genetics
  • Evolutionary Biology
  • Entomology

Background:

  • Clonal organisms, like the grain aphid (Sitobion avenae F.), reproduce asexually, leading to genetically identical offspring.
  • Maintaining a clone over many generations allows for the study of genetic stability and potential mutations in a controlled environment.

Purpose of the Study:

  • To investigate genetic stability and detect mutations within a parthenogenetically maintained clone of the grain aphid (Sitobion avenae F.) over 32 generations.
  • To characterize the nature of observed genetic changes using molecular techniques.

Main Methods:

  • A clone of Sitobion avenae F. was maintained parthenogenetically for 32 generations under constant environmental conditions.
  • Genomic DNA from individual aphids was analyzed for genetic stability using Random Amplified Polymorphic DNA-PCR (RAPD-PCR).

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  • Southern blotting and hybridization were employed to confirm the origin of observed DNA profile changes.

    • Main Results:

    • Putative germ-line mutations were observed in generation 14, and somatic mutations were detected in generations 12, 25, 27, and 29.
    • RAPD-PCR profiles showed no differences between winged and wingless morphs or in samples with symbiotic DNA.
    • Band additions identified through Southern blotting were confirmed to be of aphid origin.
    • Mutated samples exhibited unique RAPD-PCR profiles compared to other aphid clones.

    Conclusions:

    • Discernible genetic changes, including germ-line and somatic mutations, occur within an animal clonal lineage over time.
    • These findings highlight the potential for genetic variation and evolution even in asexually reproducing organisms.
    • The study underscores the importance of considering genetic dynamics in clonal systems for ecological and evolutionary studies.