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Related Experiment Videos

Neural architecture in transected rabbit sciatic nerve after prolonged nonreinnervation

J L Bradley1, D A Abernethy, R H King

  • 1Clinical Neurosciences, Royal Free Hospital School of Medicine, London, UK.

Journal of Anatomy
|September 2, 1998
PubMed
Summary

Long-term denervation of rabbit sciatic nerves leads to significant internal reorganization. Fibroblasts create collagen-rich domains, altering nerve architecture and potentially impacting chronic neuropathies.

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Area of Science:

  • Neuroscience
  • Histology
  • Regenerative Medicine

Background:

  • Peripheral nerve injury, such as transection, leads to Wallerian degeneration.
  • Schwann cells and fibroblasts play critical roles in nerve repair and degeneration.
  • Understanding the long-term structural changes in denervated nerves is crucial for treating chronic neuropathies.

Purpose of the Study:

  • To investigate the long-term structural alterations in the rabbit sciatic nerve distal to transection without reinnervation.
  • To characterize the role of fibroblasts and Schwann cells in nerve compartmentation and collagen remodeling.
  • To examine the expression of low affinity nerve growth factor receptor (NGFR) in denervated nerve tissues.

Main Methods:

  • Surgical transection of rabbit sciatic nerves with prevention of reinnervation.

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  • Histological analysis of nerve tissues at various survival periods up to 26 months.
  • Immunohistochemical staining for NGFR expression.
  • Collagen fibril characterization using electron microscopy.

    • Main Results:

    • Nerves became compartmented by fibroblast processes, forming distinct collagen domains.
    • Schwann cells within Büngner bands showed progressive atrophy but expressed NGFR up to 6 months.
    • Fibroblast processes also expressed NGFR.
    • By 26 months, acellular collagen domains with longitudinally oriented collagen fibrils replaced nerve fibers, showing varied fibril diameters.

    Conclusions:

    • Long-standing axonal loss induces a striking reorganization of peripheral nerve architecture.
    • The formation of collagen domains and fibroblast compartmentation are key long-term changes.
    • These findings may inform the interpretation of structural changes in chronic human peripheral neuropathies.