An ascidian gene encoding an SH2-domain protein is expressed in the notochord cells of the embryo

Differentiation of notochord cells in the ascidian embryo requires cell-cell interactions and signal transduction pathways. Isolation and characterization of an ascidian gene (HrSH2) from Halocynthia roretzi is reported. Sequence analysis suggests that HrSH2 encodes a polypeptide with an SH2 domain and a tyrosine kinase phosphorylation site, that are implicated in signaling pathways through tyrosine phosphorylation. Zygotic expression of HrSH2 was transient. The gene expression began at the 110-cell stage but was downregulated by the larval stage. Whole-mount in situ hybridization, taking advantage of well-known lineage, revealed that the HrSH2 transcript first appeared in primordial notochord cells as well as a few endoderm cells of the 110-cell embryo. During gastrulation and neurulation, expression in the endoderm was downregulated, and instead HrSH2 transcript became evident in notochord cells, nerve cord cells, endodermal strand cells and epidermal cells of the tail. These results suggest the possibility that HrSH2 is involved in the signal transduction pathways required for notochord formation and for differentiation of other cells in conjunction with the notochord.