Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Surfactant protein B processing in human fetal lung

S H Guttentag1, M F Beers, B M Bieler

  • 1Division of Neonatology, Department of Pediatrics, Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA.

The American Journal of Physiology
|September 5, 1998
PubMed
Summary

Surfactant protein B (SP-B) processing in human lung cells involves multiple steps. Distal processing events are key for SP-B accumulation and are regulated by development and hormones.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Retrograde signaling by a mtDNA-encoded non-coding RNA preserves mitochondrial bioenergetics.

Communications biology·2020
Same author

Organization of Neonatal Training Program Directors Council responds to the ACGME 2010 Proposed Standards.

Journal of perinatology : official journal of the California Perinatal Association·2011
Same author

Pilot trial of late booster doses of surfactant for ventilated premature infants.

Journal of perinatology : official journal of the California Perinatal Association·2011
Same author

Inhaled nitric oxide in premature infants: effect on tracheal aspirate and plasma nitric oxide metabolites.

Journal of perinatology : official journal of the California Perinatal Association·2009
Same author

SP-D-dependent regulation of NO metabolism in lipopolysaccharide-stimulated peritoneal macrophages.

Bulletin of experimental biology and medicine·2009
Same author

Susceptibility to ozone-induced airway inflammation is associated with decreased levels of surfactant protein D.

Respiratory research·2006

Area of Science:

  • Pulmonary medicine
  • Biochemistry
  • Cell biology

Background:

  • Surfactant protein B (SP-B) is crucial for lung function.
  • SP-B is synthesized from preproSP-B through intracellular processing.
  • Understanding SP-B processing is vital for respiratory health.

Purpose of the Study:

  • To characterize the intracellular processing of SP-B in human type 2 cells.
  • To investigate the developmental and hormonal regulation of SP-B processing.
  • To identify intermediates in the conversion of preproSP-B to mature SP-B.

Main Methods:

  • Human fetal lung explant culture.
  • Western blot analysis using polyclonal antibodies against SP-B and its propeptide domains.
  • Pulse-chase studies to track protein processing kinetics.

Related Experiment Videos

Main Results:

  • A novel 9-kDa intermediate, representing mature SP-B8 with a residual N-terminal peptide, was identified.
  • Pulse-chase studies confirmed a precursor-product relationship between 9-kDa and 8-kDa forms.
  • SP-B processing rate increased during type 2 cell differentiation; dexamethasone enhanced SP-B8 accumulation without altering initial processing rates.

Conclusions:

  • Cleavage of the N-terminal propeptide of proSP-B is a multistep process.
  • Later processing steps are rate-limiting for SP-B maturation.
  • SP-B processing is regulated by both developmental changes and hormonal factors like dexamethasone.