Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A mammalian scaffold complex that selectively mediates MAP kinase activation

A J Whitmarsh1, J Cavanagh, C Tournier

  • 1Program in Molecular Medicine, Department of Biochemistry and Molecular Biology, University of Massachusetts Medical School and Howard Hughes Medical Institute, Worcester, MA 01605, USA.

Science (New York, N.Y.)
|September 11, 1998
PubMed
Summary

A novel scaffold protein enhances c-Jun NH2-terminal kinase (JNK) activation. This protein interacts with key components of the JNK signaling pathway, demonstrating a role for scaffold proteins in mediating stress-induced MAP kinase activation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Identification of a universal three-body s-wave resonance in <sup>10</sup>He.

Nature communications·2026
Same author

Mass, Spectroscopy, and Two-Neutron Decay of ^{16}Be.

Physical review letters·2024
Same author

Static <i>in vitro</i> digestion model adapted to the general older adult population: an INFOGEST international consensus.

Food & function·2023
Same author

Quasifree Neutron Knockout Reaction Reveals a Small s-Orbital Component in the Borromean Nucleus ^{17}B.

Physical review letters·2021
Same author

Surface Localization of the Dineutron in ^{11}Li.

Physical review letters·2021
Same author

Fostering infant food texture acceptance: A pilot intervention promoting food texture introduction between 8 and 15 months.

Appetite·2020

Area of Science:

  • Cellular signaling pathways
  • Molecular biology
  • Stress response mechanisms

Background:

  • Mitogen-activated protein (MAP) kinases, including the c-Jun NH2-terminal kinase (JNK) group, are crucial in cellular responses to various stresses.
  • Scaffold proteins are known to organize signaling components, but their direct role in mediating MAP kinase pathway activation in mammals is not fully elucidated.

Purpose of the Study:

  • To identify and characterize a novel scaffold protein involved in the JNK signaling pathway.
  • To investigate the mechanism by which this scaffold protein influences JNK activation.
  • To establish the role of mammalian scaffold proteins in MAP kinase pathway regulation.

Main Methods:

  • Co-immunoprecipitation assays to identify protein-protein interactions.

Related Experiment Videos

  • In vitro kinase assays to measure JNK activation.
  • Expression analysis of scaffold protein and JNK pathway components.

    • Main Results:

    • A putative scaffold protein was identified that binds to mixed-lineage kinases (MLK), MKK7, and JNK.
    • This scaffold protein selectively potentiated JNK activation mediated by the MLK signaling pathway.
    • The interaction with the scaffold protein appears to be specific for the MLK-MKK7-JNK signaling cascade.

    Conclusions:

    • A mammalian scaffold protein plays a significant role in mediating the activation of the JNK MAP kinase signaling pathway.
    • Scaffold proteins can act as crucial regulators, enhancing signal transduction in response to cellular stress.
    • These findings provide new insights into the molecular mechanisms governing stress-activated JNK signaling.