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Updated: Aug 16, 2026

Determination of Fatty Acid Oxidation and Lipogenesis in Mouse Primary Hepatocytes
Published on: August 27, 2015
Fatty acid omega and (omega-1)-oxidation within intrasplenically transplanted fetal hepatocytes
1Department of Gastrointestinal Surgery, New York Medical College, Valhalla 10595, USA. kato@asahikawa-med.ac.jp
Background:
The expression and enzymatic activity of the cytochrome P450 LAomega within intrasplenically transplanted hepatocytes was investigated.
Methods:
Fetal hepatocytes were harvested from spontaneously hypertensive rats and transplanted into recipient adult spontaneously hypertensive rat spleens.
Results:
Microscopic examination revealed masses of hepatocytes in the red pulp. Immunochemical studies detected cytochrome P450 LAomega in transplanted hepatocytes by 6 and 10 weeks after transplantation. Cytochrome P450 LAomega mRNA accumulates at 6 weeks after transplantation. Cytochrome P450-arachidonic acid omega/omega-1 hydroxylase activity (formation of 20/19-hydroxyeicosatetraenoic acid) was detected at 10 weeks after transplantation.
Conclusion:
These results demonstrated that fetal hepatocytes grow in the spleen and function similarly to adult hepatocytes.
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