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Chloride channels ClC-2 and ICln mRNA expression differs in renal epithelial ontogeny
S Huber1, G Braun, B Schröppel
1Physiologisches Institut, Ludwig-Maximilians-Universität, Germany.
Abstract:
Development-dependent mRNA expression of the chloride channels ClC-2 and ICln was studied by quantitative reverse transcriptase-polymerase chain reaction in rat ureteric bud and cortical collecting duct primary monolayer cultures. Abundance of ClC-2 mRNA increased in ureteric bud cells between embryonic day 15 (E15) and E17, peaked at postnatal day 3 (P3), and was down-regulated at P7 when morphogenesis is complete, suggesting a specific embryonic function. Expression of ICln mRNA, in contrast, up-regulated continuously with development.
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