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Related Experiment Videos

Two yeast nuclear pore complex proteins involved in mRNA export form a cytoplasmically oriented subcomplex

M E Hurwitz1, C Strambio-de-Castillia, G Blobel

  • 1Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10021, USA.

Proceedings of the National Academy of Sciences of the United States of America
|September 16, 1998
PubMed
Summary

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The yeast nucleoporins Nup82 and Nup159 form a cytoplasmic complex essential for mRNA export. This complex, associated with Rss1/Gle1, does not affect nuclear import, highlighting its specific role in RNA transport.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • The nuclear pore complex (NPC) regulates transport between the nucleus and cytoplasm.
  • Specific nucleoporins mediate distinct transport functions.
  • The roles of Nup82 and Nup159 in NPC structure and function are not fully understood.

Purpose of the Study:

  • To sublocalize the yeast nucleoporin Nup82 within the NPC.
  • To investigate the interaction between Nup82 and Nup159.
  • To determine the functional significance of the Nup82-Nup159 complex in nuclear transport.

Main Methods:

  • Immunoelectron microscopy for sub-localization of Nup82.
  • In vitro binding assays to study Nup82-Nup159 interactions.
  • Depletion studies and overexpression experiments to assess functional roles.

Related Experiment Videos

  • Analysis of mRNA export and nuclear protein import.

    • Main Results:

    • Nup82 was localized to the cytoplasmic side of the NPC.
    • Nup82 directly interacts with Nup159, forming a cytoplasmic subcomplex.
    • Overexpression of Rss1/Gle1 rescues depletion of both Nup82 and Nup159.
    • Depletion of Nup82, Nup159, or Rss1/Gle1 inhibits mRNA export.
    • Depletion of Nup82 does not affect nuclear localization sequence-mediated import.

    Conclusions:

    • Yeast nucleoporins Nup159 and Nup82 form a cytoplasmically oriented subcomplex.
    • This complex is likely part of the NPC"s cytoplasmic filaments and associated with Rss1/Gle1.
    • The Nup159-Nup82 complex is crucial for mRNA export but not for classical nuclear import.