Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Base stacking: pH-mediated on-column sample concentration for capillary DNA sequencing

Y Xiong1, S R Park, H Swerdlow

  • 1Department of Human Genetics, University of Utah, Salt Lake City 84112-9454, USA.

Analytical Chemistry
|September 16, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Research progress on the impact of public health and social measures on influenza during the COVID-19 pandemic].

Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]·2024
Same author

[Clinical analysis of adverse reactions in patients with multidrug-resistant and rifampicin-resistant pulmonary tuberculosis treated with delamanid-containing regimen].

Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases·2024
Same author

[Trends in <i>Oncomelania hupensis</i> distribution in Wuhan City from 2003 to 2022 based on the Joinpoint regression model].

Zhongguo xue xi chong bing fang zhi za zhi = Chinese journal of schistosomiasis control·2024
Same author

[Saikosaponin a alleviates pentylenetetrazol-induced acute epileptic seizures in mouse models of depression by suppressing microglia activation-mediated inflammation].

Nan fang yi ke da xue xue bao = Journal of Southern Medical University·2024
Same author

[A case report on the use of an Amplatzer duct occluder type II in the treatment of congenital intrahepatic portosystemic shunt type V combined with hepatopulmonary syndrome].

Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology·2024
Same author

Long-term prognostic value of thyroid hormones in left ventricular noncompaction.

Journal of endocrinological investigation·2024
Same journal

Machine Learning-Assisted Label-Free SERS Decoding of Mitochondrial Molecular Dynamics in Ovarian Granulosa Cells during Aging.

Analytical chemistry·2026
Same journal

Revealing the Regulatory Interplay of NHE1 mRNA and Na<sup>+</sup> in Cancer Cells Using a DNA Nanosensor.

Analytical chemistry·2026
Same journal

Towards Cellular Resolution of Tryptic Peptides in Tissue Sections by MALDI MS Imaging: A Focus on Enzyme Application and Reproducibility.

Analytical chemistry·2026
Same journal

Bioinspired Bilayer Hydrogel Colorimetric Sensor Array for Low-Temperature Food Freshness Analysis.

Analytical chemistry·2026
Same journal

Quartz Crystal Microbalance-Based Point-of-Care Testing Systems: Principles, Device Design, and Applications.

Analytical chemistry·2026
Same journal

Heterojunction Gate-Empowered OPECT Aptasensing: A Valid Protocol for Realizing High Current Gain at Low Electron Donor Dependency.

Analytical chemistry·2026
See all related articles

This study introduces an on-column DNA sample concentration technique for capillary sequencing. The base-stacking method simplifies DNA fragment analysis by allowing direct injection of unpurified sequencing products.

Area of Science:

  • Molecular Biology
  • Analytical Chemistry
  • Biotechnology

Background:

  • Capillary electrophoresis is a key technique in DNA sequencing.
  • Sample preparation often involves time-consuming purification steps.
  • Efficient sample injection is crucial for high-resolution DNA analysis.

Purpose of the Study:

  • To develop and evaluate an on-column sample concentration method for capillary DNA sequencing.
  • To enable direct injection of unpurified sequencing reaction products.
  • To improve resolution and signal strength in DNA fragment analysis.

Main Methods:

  • Electrokinetic injection of hydroxide ions for on-column concentration.
  • Utilizing a neutralization reaction to create a low-conductivity zone for field focusing.

Related Experiment Videos

  • Employing poly(dimethylacrylamide) as a separation matrix in uncoated capillaries.

    • Main Results:

    • Achieved effective DNA fragment concentration and restoration of resolution with extended injection times.
    • Generated high resolution (≥0.5) for DNA fragments up to 650 nucleotides long.
    • Demonstrated excellent resolution and signal strength compared to conventional methods.
    • Confirmed no significant capillary performance degradation over 20 sequencing runs.

    Conclusions:

    • The developed on-column base-stacking method offers a simplified and efficient approach for capillary DNA sequencing.
    • This technique eliminates the need for sample pretreatment, saving time and resources.
    • It provides robust performance with excellent resolution and signal quality, suitable for routine sequencing applications.