Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Possible stage-specific function of NF-kappaB during pre-B cell differentiation

L Guerrini1, A Molteni, F Blasi

  • 1Department of Genetics and Microbial Biology, University of Milan, Italy. guerrini@imiucca.csi.unimi.it

FEBS Letters
|September 17, 1998
PubMed
Summary

Lipopolysaccharide (LPS) drives B cell differentiation by activating NF-kappaB to initiate Ig kappa gene expression. However, this transcription factor is not required for maintaining gene expression in mature B cells.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effects of insulin therapy optimization with sensor augmented pumps on glycemic control and body composition in people with cystic fibrosis-related diabetes.

Frontiers in endocrinology·2023
Same author

Humoral and cell-mediated immune responses to BNT162b2 vaccine against SARS-CoV-2 in people with cystic fibrosis.

Vaccine·2023
Same author

Surgical management of colon cancer in ulcerative colitis patients with orthotopic liver transplant for primary sclerosing cholangitis. A systematic review.

European journal of surgical oncology : the journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·2023
Same author

Staging accuracy of MRI of the prostate with special reference to the influence of the time of last ejaculation on the detection of seminal vesicle invasion.

Clinical radiology·2023
Same author

Reduced circulating FABP2 in patients with moderate to severe COVID-19 may indicate enterocyte functional change rather than cell death.

Scientific reports·2022
Same author

Changes in exercise endurance and inspiratory capacity after lumacaftor/ivacaftor therapy in cystic fibrosis.

Pulmonology·2022

Area of Science:

  • Immunology
  • Molecular Biology
  • Cell Differentiation

Background:

  • Lipopolysaccharide (LPS) is a key inducer of B cell differentiation.
  • The murine pre-B cell line 70Z/3 serves as a model for pre-B to B cell differentiation.
  • Nuclear factor-kappaB (NF-kappaB) is known to be essential for initiating immunoglobulin kappa (Ig kappa) gene expression.

Purpose of the Study:

  • To investigate the role of NF-kappaB in the late stages of LPS-induced B cell differentiation.
  • To understand the mechanism regulating Ig kappa gene transcription maintenance in mature B cells.

Main Methods:

  • Utilized the 70Z/3 murine pre-B cell line.
  • Administered LPS treatment for extended periods (up to 96 hours).
  • Assessed NF-kappaB DNA binding activity, IkappaB alpha nuclear accumulation, and nuclear extract treatments.

Related Experiment Videos

Main Results:

  • NF-kappaB binding activity was induced early by LPS but diminished after 84 hours.
  • IkappaB alpha accumulated in the nucleus, correlating with NF-kappaB activity loss.
  • Sodium deoxycholate treatment restored NF-kappaB binding activity in late-stage cells.

Conclusions:

  • NF-kappaB is crucial for initiating Ig kappa gene transcription during early B cell differentiation.
  • NF-kappaB is not required for maintaining Ig kappa gene transcription in differentiated B cells.
  • Mechanisms involving IkappaB alpha nuclear localization and potential post-translational modifications regulate late-stage NF-kappaB activity.