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Unique morphogenesis in Saccharomyces cerevisiae strain GS1731
V K Singh1, D A McCracken, M J Nadakavukaren
1Department of Biological Sciences, Illinois State University, Normal 61790-4120, USA.
FEMS Microbiology Letters
|September 19, 1998
Summary
Saccharomyces cerevisiae mother cells can form multiple buds simultaneously during early growth phases. This multiple budding capability, involving up to three buds, was observed using advanced microscopy techniques.
Area of Science:
- Cell biology
- Microbiology
- Yeast genetics
Background:
- Budding is a primary mode of cell division in Saccharomyces cerevisiae.
- Understanding the regulation of budding is crucial for cell cycle studies.
Purpose of the Study:
- To investigate the phenomenon of multiple budding in Saccharomyces cerevisiae.
- To characterize the cellular structures and processes involved in multiple budding.
Main Methods:
- Light and scanning electron microscopy to visualize bud formation.
- Immunofluorescence microscopy to study microtubule organization.
- DAPI staining to analyze nuclear content.
- Hydroxyurea treatment to induce synchronized growth.
Main Results:
- 50-60% of budded cells showed multiple buds during early growth phases.
- Mother cells could initiate up to three simultaneous bud developments.
- Multiple buds could develop concurrently or sequentially.
- Multiply budded cells exhibited multiple cytoplasmic microtubule bundles.
- Some unbudded mother cells were multinucleate and underwent normal cytokinesis.
Conclusions:
- Saccharomyces cerevisiae exhibits a capacity for multiple simultaneous bud formation.
- This process is associated with multiple microtubule organizing centers and nuclear division.
- The frequency of multiple budding varies with growth phase.